Regulatory

Part:BBa_K1824555:Design

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-04)


Site-directed mutated pLux that permits strong validity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the consideration of binding efficency of LuxR-AHL complex to the Lux box that embedded in pLux. Zhang only mutated -10 region of the original pLux.


Source

The DNA sequences of this part was provided by Haoqian Zhang, Peking University.

References